Electrophoretic Separation of X- and Y-Chromo-some-Bearing Sperm in Human Semen
نویسندگان
چکیده
منابع مشابه
The Effect of Diffrent Factors on X- and Y Bearing Sperm in Bull Semen
Background: Newborn gender ratio is one of the most important factors in livestock industry. This ratio has affected on economic condition and controls sex-linked genetically transferred diseases. Offspring sex ratio is affected by such various factors. One of these factors is the Y to X bearing sperm ratio in normal semen that is called primary sex ratio, season and individual differences are ...
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Introduction: To evaluate the 8 layer discontinues puresperm gradient in separation of human spermatozoa according to sex chromosomes by fluorescent in situ hybridization (FISH). Material and Methods: This study was carried out on three patient referring to Royan fertility and infertility center. Semen analysis was assessed according to WHO criteria. Each sample was divided into two aliquots, ...
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Studies showed that assisted reproductive technology changed the sex ratio at birth, but the effect of semen processing (swim-up technique and density-gradient separation) on enriching either Xor Y-bearing sperm is always controversial. We recruited 30 healthy males and their semen samples were obtained by masturbation after sexual abstinence for 2-7 days. Each sample was divided into 3 aliquot...
متن کاملImproved Flow Cytometric Sorting of X- and Y-Chromosome Bearing Sperm: Substantial Increase in Yield of Sexed Semen
The yield of flow cytometric sorted Xand Y-chromosome-bearing sperm in a given time period is an important factor in the strategies used for fertilization and the production of sex-preselected offspring. This yield is dependent on the efficiency with which the modified flow cytometer/cell sorter analyzes the DNA of spermatozoa. The efficiency is directly related to the number of sperm with the ...
متن کاملthe effects of follicular fluid on human x y bearing sperm ratio by fluorescent in situ hybridization (fish)
background: some evidences showed that the secretion of uterine tube vagina and follicular fluid (ff) affects x and y-chromosome populations. sperm selection with x or y chromosome can added to oocyte for gender desired. the isolation of x y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. the objective of this study was to find if the f...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1974
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.113.273